Applications Of Flow Cytometry In Stem Cell Research And Tissue Regeneration

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Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Author : Awtar Krishan
Publisher : John Wiley & Sons
ISBN 13 : 1118148061
Total Pages : 317 pages
Book Rating : 4.1/5 (181 download)

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Book Synopsis Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration by : Awtar Krishan

Download or read book Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration written by Awtar Krishan and published by John Wiley & Sons. This book was released on 2011-06-09 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.

Stem Cell-based Tissue Repair

Author : Raphael Gorodetsky
Publisher : Royal Society of Chemistry
ISBN 13 : 1849730016
Total Pages : 405 pages
Book Rating : 4.8/5 (497 download)

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Book Synopsis Stem Cell-based Tissue Repair by : Raphael Gorodetsky

Download or read book Stem Cell-based Tissue Repair written by Raphael Gorodetsky and published by Royal Society of Chemistry. This book was released on 2011 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book by leading experts reviews the key therapeutic issues related to stem cell technology.

Working with Stem Cells

Author : Henning Ulrich
Publisher : Springer
ISBN 13 : 3319305824
Total Pages : 381 pages
Book Rating : 4.3/5 (193 download)

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Book Synopsis Working with Stem Cells by : Henning Ulrich

Download or read book Working with Stem Cells written by Henning Ulrich and published by Springer. This book was released on 2016-08-04 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: The content covered encompass not only the theoretic and methodological aspects for culturing human pluripotent stem cells, but also the establishment of induced pluripotent stem cells and their differentiation into specific cellular subtypes. This book has the most updated methods and technologies in an accessible and clear language, presenting protocols developed and optimized by researchers that use them every day. Stem cell research is a growing field that offers the potential to comprehend mechanisms of neural development and tissue regeneration and maintenance as well as the development-related human diseases and the establishment of novel therapies. Questions of basic biology can also be exploited by this cutting-edge approach.

Stem Cells in Clinical Practice and Tissue Engineering

Author : Rakesh Sharma
Publisher : BoD – Books on Demand
ISBN 13 : 1789230101
Total Pages : 353 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Stem Cells in Clinical Practice and Tissue Engineering by : Rakesh Sharma

Download or read book Stem Cells in Clinical Practice and Tissue Engineering written by Rakesh Sharma and published by BoD – Books on Demand. This book was released on 2018-05-02 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem Cells in Clinical Practice and Tissue Engineering is a concise book on applied methods of stem cell differentiation and optimization using tissue engineering methods. These methods offer immediate use in clinical regenerative medicine. The present volume will serve the purpose of applied stem cell differentiation optimization methods in clinical research projects, as well as be useful to relatively experienced stem cell scientists and clinicians who might wish to develop their stem cell clinical centers or research labs further. Chapters are arranged in the order of basic concepts of stem cell differentiation, clinical applications of pluripotent stem cells in skin, cardiac, bone, dental, obesity centers, followed by tissue engineering, new materials used, and overall evaluation with their permitted legal status.

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Author : Awtar Krishan
Publisher : John Wiley and Sons
ISBN 13 : 1118125851
Total Pages : 312 pages
Book Rating : 4.1/5 (181 download)

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Book Synopsis Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration by : Awtar Krishan

Download or read book Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration written by Awtar Krishan and published by John Wiley and Sons. This book was released on 2011-05-12 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.

Embryonic Stem Cell Protocols

Author : Kursad Turksen
Publisher : Springer Science & Business Media
ISBN 13 : 1597450375
Total Pages : 504 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Embryonic Stem Cell Protocols by : Kursad Turksen

Download or read book Embryonic Stem Cell Protocols written by Kursad Turksen and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Applications of Flow Cytometry in Blood and Marrow Stem Cell Transplantation

Author : International Society for Hematotherapy and Graft Engineering
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (788 download)

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Book Synopsis Applications of Flow Cytometry in Blood and Marrow Stem Cell Transplantation by : International Society for Hematotherapy and Graft Engineering

Download or read book Applications of Flow Cytometry in Blood and Marrow Stem Cell Transplantation written by International Society for Hematotherapy and Graft Engineering and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

Author : Anna Porwit
Publisher : Cambridge University Press
ISBN 13 : 1108548830
Total Pages : 248 pages
Book Rating : 4.1/5 (85 download)

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Book Synopsis Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by : Anna Porwit

Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.

Neural Surface Antigens

Author : Jan Pruszak
Publisher : Academic Press
ISBN 13 : 0128011262
Total Pages : 253 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Neural Surface Antigens by : Jan Pruszak

Download or read book Neural Surface Antigens written by Jan Pruszak and published by Academic Press. This book was released on 2015-03-23 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neural Surface Antigens: From Basic Biology towards Biomedical Applications focuses on the functionalrole of surface molecules in neural development, stem cell research, and translational biomedical paradigms.With an emphasis on human and rodent model systems, this reference covers fundamentals of neural stemcell biology and flow cytometric methodology. Addressing cell biologists as well as clinicians working in theneurosciences, the book was conceived by an international panel of experts to cover a vast array of particularsurface antigen families and subtypes. It provides insight into the basic biology and functional mechanisms ofneural cell surface signaling molecules influencing mammalian development, regeneration, and treatments. Introduces early phase clinical trials of neural stem cells Outlines characterization of surface molecule expression and methods for isolation which open unprecedented opportunities for functional study, quantitation & diagnostics Highlights the role of stem cells in neural surface antigen and biomarker analysis and applications

Stem Cells in Regenerative Medicine

Author : Julie Audet
Publisher : Humana Press
ISBN 13 : 9781617377846
Total Pages : 0 pages
Book Rating : 4.3/5 (778 download)

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Book Synopsis Stem Cells in Regenerative Medicine by : Julie Audet

Download or read book Stem Cells in Regenerative Medicine written by Julie Audet and published by Humana Press. This book was released on 2011-01-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular BiologyTM series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.

Translational Stem Cell Research

Author : Kristina Hug
Publisher : Springer Science & Business Media
ISBN 13 : 1607619598
Total Pages : 463 pages
Book Rating : 4.6/5 (76 download)

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Book Synopsis Translational Stem Cell Research by : Kristina Hug

Download or read book Translational Stem Cell Research written by Kristina Hug and published by Springer Science & Business Media. This book was released on 2010-12-25 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: For many years, the ethical discussion surrounding human embryonic stem cell research has focused on the moral status of the embryo. This text takes a wider moral berth and focuses on numerous ethical, legal and social aspects involved in translating the results of stem cell research into diagnostic and therapeutic applications. Translational Stem Cell Research is broken into ten sections. It opens with an overview of the latest in stem cell research, focusing on specific diseases and the treatment of burn victims. Part II discusses the issues involved in the many steps from bench to bedside, ranging from first research in vitro to clinical trials. Part III covers scientific, regulatory and ethical challenges to basic research, and Part IV details issues regarding stem cell banks. Part V explores ethical, economic and strategic issues involved in collaboration between universities and industry, and Part VI addresses legal problems raised by patents on human stem-cell based inventions plus the extent to which there can be technological solutions to a moral dilemma. Part VII presents imaginative ways of communicating research to the general public and how to create conditions for a constructive dialogue. Part VIII probes psychosocial and cultural factors affecting judgment and decisions about translational stem cell research, and Part IX explores problems and procedures raised by an examination of the evaluation of stem cell research projects in research ethics committees. The book closes with a look into the future of translational stem cell research and stem cell-based therapeutic applications.

Principles of Stem Cell Biology and Cancer

Author : Tarik Regad
Publisher : John Wiley & Sons
ISBN 13 : 1118670604
Total Pages : 376 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Principles of Stem Cell Biology and Cancer by : Tarik Regad

Download or read book Principles of Stem Cell Biology and Cancer written by Tarik Regad and published by John Wiley & Sons. This book was released on 2015-04-01 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Stem Cell Biology and Cancer: Future Applications and Therapeutics Tarik Regad, The John van Geest Cancer Research Centre, Nottingham Trent University, UK, Thomas J. Sayers, Centre for Cancer Research, National Cancer Institute, Frederick, USA and Robert Rees The John van Geest Cancer Research Centre, Nottingham Trent University, UK The field of cancer stem cells is expanding rapidly, with many groups focusing on isolating and identifying cancer stem cell populations. Although some progress has been made developing efficient cancer therapies, targeting cancer stem cells remains one of the important challenges facing the growing stem cell research community. Principles of Stem Cell Biology and Cancer brings together original contributions from international experts in the field to present the very latest information linking stem cell biology and cancer. Divided into two parts, the book begins with a detailed introduction to stem cell biology with a focus on the characterization of these cells, progress that has been made in their identification, as well as future therapeutic applications of stem cells. The second part focuses on cancer stem cells and their role in cancer development, progression and chemo-resistance. This section of the book includes an overview of recent progress concerning therapies targeting cancer stem cells. Features: An authoritative introduction to the link between stem cell biology and cancer. Includes contributions from leading international experts in the field. Well-illustrated with full colour figures throughout. This book will prove an invaluable resource for basic and applied researchers and clinicians working on the development of new cancer treatments and therapies, providing a timely publication of high quality reviews outlining the current progress and exciting future possibilities for stem cell research.

Deconstructing the Stem Cell Niche

Author : Karen Havenstrite
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (745 download)

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Book Synopsis Deconstructing the Stem Cell Niche by : Karen Havenstrite

Download or read book Deconstructing the Stem Cell Niche written by Karen Havenstrite and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The ultimate goal of regenerative medicine is to repair tissues damaged by aging, injury or disease. Tissue-specific adult stem cells constitute a reservoir of cells in postnatal tissue that have the remarkable capacity to proliferate and repair tissue damage because they can both self-renew, or produce more stem cells, and differentiate into mature cells. Adult stem cells have been identified in a variety of tissues, including blood, brain, skin, intestine, and muscle and methods exist to prospectively isolate these populations by flow cytometry. Upon transplantation, they possess an extraordinary ability to contribute extensively to tissue regeneration. Notably, adult stem cells are a relatively rare cell population and methods to propagate and expand these cell types in culture without loss of regenerative capacity are lacking, a hurdle to their clinical use. In vivo, stem cells reside in an instructive microenvironment, or niche, which serves to regulate fate decisions, including quiescence, self-renewal, and differentiation. Given the complexity of their native environment, it is not surprising that upon removal from their niche they rapidly lose regenerative capacity. While the role of biochemical signals in regulating stem cell fate and function has been widely explored, the effects of biophysical signals have not been discerned. To elucidate the role of matrix elasticity in regulating adult stem cell fate, we first design a biomimetic hydrogel culture platform to mimic tissue elasticity and physiologic presentation of biochemical cues. Adapting a previously described conjugate addition reaction, poly(ethylene glycol) hydrogel substrates are fabricated which have a Young's modulus that is tunable in the physiologic range (1-50kPa). Gels are designed to have limited post-polymerization swelling to enable constant density of tethered biological ligands. Utilizing this tunable hydrogel culture platform, we provide the first definitive evidence that matrix elasticity regulates adult stem cell self-renewal in culture. Using a combination of culture studies and in vivo functional assays in mice, we demonstrate that substrate rigidity profoundly impacts the self-renewal potential of tissue-specific adult stem cells isolated from skeletal muscle and bone marrow. In contrast with rigid tissue culture plastic in which regenerative potential is lost, we demonstrate that culture on a pliant hydrogel substrate maintains the 'stemness' of muscle stem cells (MuSCs) and hematopoetic stem cells (HSCs). Further, we describe a novel in vivo screen and identify an extracellular matrix molecule which, in conjunction with soft hydrogel, has a previously unrecognized role in regulating HSC fate. These studies demonstrate that recapitulating tissue rigidity, a key component of the in vivo microenvironment, enables propagation of functional adult stem cells in culture for the first time. We expect these experimental approaches will be broadly applicable to other adult stem cell types and ultimately will profoundly impact regenerative medicine by enabling generation of functional stem cell populations for use in clinical cell-based therapies.

Frontiers in Stem Cell and Regenerative Medicine Research: Volume 10

Author : Atta-ur-Rahman
Publisher : Bentham Science Publishers
ISBN 13 : 9811464685
Total Pages : 172 pages
Book Rating : 4.8/5 (114 download)

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Book Synopsis Frontiers in Stem Cell and Regenerative Medicine Research: Volume 10 by : Atta-ur-Rahman

Download or read book Frontiers in Stem Cell and Regenerative Medicine Research: Volume 10 written by Atta-ur-Rahman and published by Bentham Science Publishers. This book was released on 2022-03-03 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: Frontiers in Stem Cell and Regenerative Medicine Research Volume 10 [Edited for Volume 10] Stem cell and regenerative medicine research is an important area of clinical research which promises to change the face of medicine as it will be practiced in the years to come. Challenges in the 21st century to combat diseases such as cancer, Alzheimer’s disease and retinal disorders, among others, may well be addressed employing stem cell therapies and tissue regeneration techniques. Frontiers in Stem Cell and Regenerative Medicine Research brings updates on multidisciplinary topics relevant to stem cell research and their application in regenerative medicine. The series is essential reading for researchers seeking updates in stem cell therapeutics and regenerative medicine. Volume 10 includes 5 chapters on these topics: -Novel drugs and their stem cell-based targets for osteoporosis: challenges and proceedings -The role of cancer stem cells in disease progression and therapy resistance -Stem cells from human exfoliated deciduous teeth in tissue regeneration -The fate of toxicological studies: from animal models to stem cell-based methods -Effect of material properties on differentiation of mesenchymal stem cells

Epidermal Cells

Author : Kursad Turksen
Publisher : Springer Science & Business Media
ISBN 13 : 1592598307
Total Pages : 482 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Epidermal Cells by : Kursad Turksen

Download or read book Epidermal Cells written by Kursad Turksen and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.

Stem Cell Anthology

Author :
Publisher : Academic Press
ISBN 13 : 0123756839
Total Pages : 425 pages
Book Rating : 4.1/5 (237 download)

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Book Synopsis Stem Cell Anthology by :

Download or read book Stem Cell Anthology written by and published by Academic Press. This book was released on 2009-10-22 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fields of stem cell research, regenerative medicine, tissue engineering, and cloning are very closely related. It is important for researchers in each of these disciplines to be aware of the methods and principles in the others. Elsevier publishes some of the highest individual references in these areas. Bringing together the principles, applications, and basic understanding in these related areas of science will provide a new reference which is serve the needs of a variety of researchers. Edited by Dr. Bruce Carlson, Stem Cell Anthology will be valuable to researchers and students who need to save time and link concepts to principles, applications, and methods in order to work more effectively and see links for potential collaborations. Includes a collection of chapters by leaders in the stem cell field including the first researchers to discover iPS cells and multiple Nobel Laureates Provides the most detailed introduction to basic properties of major embryonic and adult stem cells by highlighting breakthrough discoveries in the nervous system, spinal cord, heart, pancreas, epidermis, musculo-skeletal, retina - leading areas of stem cell research in human application Details technical laboratory set up for practitioners, technicians, and administrators

Adipose-Derived Stem Cells

Author : Jeffrey M. Gimble
Publisher : Humana Press
ISBN 13 : 9781617379611
Total Pages : 474 pages
Book Rating : 4.3/5 (796 download)

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Book Synopsis Adipose-Derived Stem Cells by : Jeffrey M. Gimble

Download or read book Adipose-Derived Stem Cells written by Jeffrey M. Gimble and published by Humana Press. This book was released on 2011-08-24 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.