Application of Biofilms in Applied Microbiology

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Publisher : Academic Press
ISBN 13 : 0323905250
Total Pages : 308 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Application of Biofilms in Applied Microbiology by : Maulin P Shah

Download or read book Application of Biofilms in Applied Microbiology written by Maulin P Shah and published by Academic Press. This book was released on 2022-08-09 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Application of Biofilms in Applied Microbiology gives a complete overview on the structure, physiology and application of biofilms produced by microbes, along with their potential application in biotechnology. Sections cover new technologies for biofilm study, physiology of microorganisms in biofilms, bacterial biofilms, biofilm development, and fungal biofilms, summarizing various technologies available for biofilm study. Subsequent chapters describe biofilm developments with Bacillus subtillis, Escherichia coli, and Pseudomonas putida, along with several chapters on the study of microbial biofilm and their advantages and disadvantages in the area of environmental biotechnology. The book closes with a chapter on the rapid development of new sequencing technologies and the use of metagenomics, thus revealing the great diversity of microbial life and enabling the emergence of a new perspective on population dynamics. Summarizes various technologies available for biofilm study Describes the physiological study of bacteria, fungi and algae present in biofilms Provides the potential parameters on biofilm development Gives insights on the ability to construct and maintain a structured multicellular bacterial community that critically depends on the production of extracellular matrix components Reveals the rapid development of new sequencing technologies and the use of metagenomics, the great diversity of microbial life, and the emergence of a new perspective on population dynamics

Microbial Biofilms in Bioremediation and Wastewater Treatment

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Publisher : CRC Press
ISBN 13 : 1351857312
Total Pages : 439 pages
Book Rating : 4.3/5 (518 download)

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Book Synopsis Microbial Biofilms in Bioremediation and Wastewater Treatment by : Y.V. Nancharaiah

Download or read book Microbial Biofilms in Bioremediation and Wastewater Treatment written by Y.V. Nancharaiah and published by CRC Press. This book was released on 2019-10-18 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofilms represent the natural living style of microbial communities and play a pivotal role in biogeochemical cycles and natural attenuation. Biofilms can be engineered for biodegradation and biotransformation of organic and inorganic contaminants, for both in situ bioremediation and ex situ treatment in bioreactors. This book focuses on microbial biofilms and their potential technological applications for sustainable development. It covers recent advances in biofilm technologies for contaminant remediation coupled to recovery of resources and serves as a complete reference on the science and technology behind biofilm mediated bioremediation and wastewater treatment.

Biofilms

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Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 824 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Biofilms by : William G. Characklis

Download or read book Biofilms written by William G. Characklis and published by Wiley-Interscience. This book was released on 1990-01-16 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: A state-of-the-art review of biofilm science and technology, incorporating approaches from microbiology, physical chemistry, environmental engineering and chemical engineering. The book describes the physical and chemical properties of biofilms and the processes by which they accumulate.

Microbial Biofilms

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Publisher : CRC Press
ISBN 13 : 1000573796
Total Pages : 360 pages
Book Rating : 4.0/5 (5 download)

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Book Synopsis Microbial Biofilms by : Naga Raju Maddela

Download or read book Microbial Biofilms written by Naga Raju Maddela and published by CRC Press. This book was released on 2022-05-17 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial biofilms have both positive and negative effects. This book considers new ways of controlling environmental microbial biofilm such as using phages, nanotechnology, and newly discovered microbial enzymes. A team of contributors shares current, relevant and original research to add weight and recognition to the book. Also, each chapter provides enlightening and relevant tabular information, charts, and illustrations. The book is, therefore, informative, precise, useful and easily digested by users.

Advances in Biofilm Science and Engineering

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Publisher : Cytergy
ISBN 13 : 0974180211
Total Pages : 282 pages
Book Rating : 4.9/5 (741 download)

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Book Synopsis Advances in Biofilm Science and Engineering by : Ryan Jordan

Download or read book Advances in Biofilm Science and Engineering written by Ryan Jordan and published by Cytergy. This book was released on 2004 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial biofilms provide challenges in many fields of science, technology, and engineering. Advances in Biofilm Science and Engineering offers a state of the art review of new science about microbial biofilms in the areas of biotechnology, healthcare, and medicine.The purpose of this text is to strengthen [the biofilm] information nucleus by offering six core discussions of established concepts in biofilm science. The audience for this text is not just academic; it is trade. This book is for physicians, industrial microbiologists, product managers, process engineers, policy makers and public health officials. [The] authors represent some of the brightest researchers in the field of biofilm science and engineering and comprise a small subset of the research community with a passion for bringing biofilm information to industrial and medical trade professionals.Table of ContentsForward, by Ryan JordanChapter 1. The History and Use of Heterotrophic Plate Counts in Water Systems, by Anne Camper (Center for Biofilm Engineering, Montana State University)Chapter 2. Methods for Biofilm Study and Assays for Biofilm Susceptibility and Target Recognition: Approaches to Deal with the Biofilm Mode of Life, by Howard Ceri, Merle E. Olson, D. Storey, and D. Morck (Biofilm Research Group and Biological Sciences, University of Calgary)Chapter 3. Extracellular Polymeric Substances, by H.C. Flemming and J. Wingender (Gerhard-Mercator-University Duisburg Insitute of Interfacial Biotechnology Department of Aquatic Microbiology, Duisburg, Germany)Chapter 4. Biofilms and Antimicrobial Resistance: Beyond Physiological and Chemical Elements, by Peter Gilbert and Andrew McBain (School of Pharmacy and Pharmaceutical Sciences, University of Manchester)Chapter 5. Diverse Mechanisms of Biofilm Formation, by Lisa Friedman and Roberto Kolter (Department of Microbiology and Molecular Genetics, Harvard Medical School)Chapter 6. Biofilm Formation and Control in Food Processing Environments, by Amy C. Lee Wong (Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin-Madison)

Understanding Microbial Biofilms

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Publisher : Elsevier
ISBN 13 : 0323983081
Total Pages : 774 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Understanding Microbial Biofilms by : Surajit Das

Download or read book Understanding Microbial Biofilms written by Surajit Das and published by Elsevier. This book was released on 2022-10-27 with total page 774 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding Microbial Biofilms: Fundamentals to Applications focuses on the microbial biofilms of different environments. The book provides a comprehensive overview of the fundamental aspects of microbial biofilms, their existence in nature, their significance, and the different clinical and environmental problems associated with them. The book covers both the fundamentals and applications of microbial biofilms, with chapters on the introduction to the microbial community and its architecture, physiology, mechanisms and imaging of biofilms in nature and fungal, algal, and bacillus biofilm control. In addition, the book highlights the molecular and biochemical aspects of bacterial biofilms, providing a compilation of chapters on the bacterial community and communication from different environments. Finally, the book covers recent advancements in various aspects of microbial biofilms including the chapters on their biotechnological applications. All the chapters are written by experts who have been working on different aspects of microbial biofilms. Illustrates fundamental aspects surrounding microbial biofilms, along with recent advancements Provides an overview on the principal aspects of biofilms, i.e., formation, regulation, distribution, control, and application Updates on the progress on biofilm regulation through ‘omics’ Serves as a classical manual for all researchers, academicians, and students who would want complete insights on biofilms in a single resource Covers all recent advancements and amendments on microbial biofilms

Biofilms in Human Diseases: Treatment and Control

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Publisher : Springer Nature
ISBN 13 : 3030307573
Total Pages : 318 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Biofilms in Human Diseases: Treatment and Control by : Sunil Kumar

Download or read book Biofilms in Human Diseases: Treatment and Control written by Sunil Kumar and published by Springer Nature. This book was released on 2019-11-19 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights treatment strategies for bacterial biofilms in connection with a variety of human diseases. In particular, it reviews bacterial biofilm formation and its mechanism. Topics covered include biofilms in human health, the role of biofilms in mediating human diseases, and methods for testing bacterial biofilms. Further sections concentrate on biofilm-mediated diseases in different parts of the human gastrointestinal tract, while therapeutic strategies for biofilm control and natural agents that disrupt bacterial biofilms are also covered. Readers will also find the latest advances in probiotics and biofilms, as well as the use of probiotics to counteract biofilm-associated infections. Biofilms and antimicrobial resistance are discussed. Subsequent chapters address the management of inflammatory bowel disease via probiotics biofilms, as well as the role of probiotics bacteria in the treatment of human diseases associated with bacterial biofilms. The book is chiefly intended for clinicians/scientists in the fields of medical microbiology, applied microbiology, biochemistry, and biotechnology.

Microbial Biofilms

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Publisher :
ISBN 13 : 9781912530502
Total Pages : pages
Book Rating : 4.5/5 (35 download)

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Book Synopsis Microbial Biofilms by : Gavin Lear

Download or read book Microbial Biofilms written by Gavin Lear and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microbial Biofilms

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Publisher : BoD – Books on Demand
ISBN 13 : 9535124358
Total Pages : 526 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Microbial Biofilms by : Dharumadurai Dhanasekaran

Download or read book Microbial Biofilms written by Dharumadurai Dhanasekaran and published by BoD – Books on Demand. This book was released on 2016-07-13 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the book Microbial Biofilms: Importance and applications, eminent scientists provide an up-to-date review of the present and future trends on biofilm-related research. This book is divided with four subdivisions as biofilm fundamentals, applications, health aspects, and their control. Moreover, this book also provides a comprehensive account on microbial interactions in biofilms, pyocyanin, and extracellular DNA in facilitating Pseudomonas aeruginosa biofilm formation, atomic force microscopic studies of biofilms, and biofilms in beverage industry. The book comprises a total of 21 chapters from valued contributions from world leading experts in Australia, Bulgaria, Canada, China, Serbia, Germany, Italy, Japan, the United Kingdom, the Kingdom of Saudi Arabia, Republic of Korea, Mexico, Poland, Portugal, and Turkey. This book may be used as a text or reference for everyone interested in biofilms and their applications. It is also highly recommended for environmental microbiologists, soil scientists, medical microbiologists, bioremediation experts, and microbiologists working in biocorrosion, biofouling, biodegradation, water microbiology, quorum sensing, and many other related areas. Scientists in academia, research laboratories, and industry will also find it of interest.

Biofilms in the Food Environment

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Publisher : John Wiley & Sons
ISBN 13 : 1118864069
Total Pages : 313 pages
Book Rating : 4.1/5 (188 download)

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Book Synopsis Biofilms in the Food Environment by : Anthony L. Pometto III

Download or read book Biofilms in the Food Environment written by Anthony L. Pometto III and published by John Wiley & Sons. This book was released on 2015-08-24 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: In nature, microorganisms are generally found attached to surfaces as biofilms such as dust, insects, plants, animals and rocks, rather than suspended in solution. Once a biofilm is developed, other microorganisms are free to attach and benefit from this microbial community. The food industry, which has a rich supply of nutrients, solid surfaces, and raw materials constantly entering and moving through the facility, is an ideal environment for biofilm development, which can potentially protect food pathogens from sanitizers and result in the spread of foodborne illness. Biofilms in the Food Environment is designed to provide researchers in academia, federal research labs, and industry with an understanding of the impact, control, and hurdles of biofilms in the food environment. Key to biofilm control is an understanding of its development. The goal of this 2nd edition is to expand and complement the topics presented in the original book. Readers will find: The first comprehensive review of biofilm development by Campylobacter jejuni An up-date on the resistance of Listeria monocytogenes to sanitizing agents, which continues to be a major concern to the food industry An account of biofilms associated with various food groups such as dairy, meat, vegetables and fruit is of global concern A description of two novel methods to control biofilms in the food environment: bio-nanoparticle technology and bacteriophage Biofilms are not always a problem: sometimes they even desirable. In the human gut they are essential to our survival and provide access to some key nutrients from the food we consume. The authors provide up-date information on the use of biofilms for the production of value-added products via microbial fermentations. Biofilms cannot be ignored when addressing a foodborne outbreak. All the authors for each chapter are experts in their field of research. The Editors hope is that this second edition will provide the bases and understanding for much needed future research in the critical area of Biofilm in Food Environment.

Biofilms in the Dairy Industry

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Publisher : John Wiley & Sons
ISBN 13 : 1118876237
Total Pages : 292 pages
Book Rating : 4.1/5 (188 download)

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Book Synopsis Biofilms in the Dairy Industry by : Koon Hoong Teh

Download or read book Biofilms in the Dairy Industry written by Koon Hoong Teh and published by John Wiley & Sons. This book was released on 2015-06-29 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the formation and impacts of biofilms on dairy manufacturing have been studied extensively, from the effects of microbial enzymes produced during transportation of raw milk to the mechanisms of biofilm formation by thermophilic spore-forming bacteria. The dairy industry now has a better understanding of biofilms and of approaches that may be adopted to reduce the impacts that biofilms have on manufacturing efficiencies and the quality of dairy products. Biofilms in the Dairy Industry provides a comprehensive overview of biofilm-related issues facing the dairy sector. The book is a cornerstone for a better understanding of the current science and of ways to reduce the occurrence of biofilms associated with dairy manufacturing. The introductory section covers the definition and basic concepts of biofilm formation and development, and provides an overview of problems caused by the occurrence of biofilms along the dairy manufacturing chain. The second section of the book focuses on specific biofilm-related issues, including the quality of raw milk influenced by biofilms, biofilm formation by thermoduric streptococci and thermophilic spore-forming bacteria in dairy manufacturing plants, the presence of pathogens in biofilms, and biofilms associated with dairy waste effluent. The final section of the book looks at the application of modelling approaches to control biofilms. Potential solutions for reducing contamination throughout the dairy manufacturing chain are also presented. Essential to professionals in the global dairy sector, Biofilms in the Dairy Industry will be of great interest to anyone in the food and beverage, academic and government sectors. This text is specifically targeted at dairy professionals who aim to improve the quality and consistency of dairy products and improve the efficiency of dairy product manufacture through optimizing the use of dairy manufacturing plant and reducing operating costs.

The Science and Applications of Synthetic and Systems Biology

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Publisher : National Academies Press
ISBN 13 : 0309219396
Total Pages : 570 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis The Science and Applications of Synthetic and Systems Biology by : Institute of Medicine

Download or read book The Science and Applications of Synthetic and Systems Biology written by Institute of Medicine and published by National Academies Press. This book was released on 2011-12-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.

Advances in Microbial Biotechnology

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Publisher : CRC Press
ISBN 13 : 1351248898
Total Pages : 536 pages
Book Rating : 4.3/5 (512 download)

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Book Synopsis Advances in Microbial Biotechnology by : Pradeep Kumar, PhD.

Download or read book Advances in Microbial Biotechnology written by Pradeep Kumar, PhD. and published by CRC Press. This book was released on 2018-10-03 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last few decades, the rapid and vast development of advanced microbial bioresources and metagenomics techniques has completely transformed the field of microbial biotechnology. Our understanding of microbial diversity, evolutionary biology, and microbial interaction with their animal and plant hosts at molecular level has been revolutionized with an abundance of new research. This new volume, Advances in Microbial Biotechnology: Current Trends and Future Prospect, focuses on the application of microorganisms for several purposes: for plant protection and improvement, for environmental remediation purposes, and for the improvement of human health. Various applications of microorganisms are covered broadly and have been appropriately reflected in depth in different chapters. The book is divided into four major sections: applied microbiology in agriculture microbes in the environment microbes in human health microbes in nanotechnology The book provides insight into the diverse microorganisms that have been explored and exploited in the development of various applications for agricultural improvements. The book also looks at the application of microbes for the removal of pollutants and the recovery of metals and oils. Also discussed is the detection and exploitation of microorganisms in the diagnosis of human diseases, providing possible holistic approaches to health. This new volume will provide a wealth of information on new research on the application of microbial biotechnology today.

Biofilms in Medicine, Industry and Environmental Biotechnology

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Publisher : IWA Publishing
ISBN 13 : 1843390191
Total Pages : 634 pages
Book Rating : 4.8/5 (433 download)

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Book Synopsis Biofilms in Medicine, Industry and Environmental Biotechnology by : Piet Lens

Download or read book Biofilms in Medicine, Industry and Environmental Biotechnology written by Piet Lens and published by IWA Publishing. This book was released on 2003-04-30 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofilms are of great practical importance for beneficial technologies such as water and wastewater treatment and bioremediation of groundwater and soil. In other settings biofilms cause severe problems, for example in 65% of bacterial infections currently treated by clinicians (particularly those associated with prosthetics and implants), accelerated corrosion in industrial systems, oil souring and biofouling. Until recently, the structure and function of biofilms could only be inferred from gross measures of biomass and metabolic activity. This limitation meant that investigators involved in biofilm research and application had only a crude understanding of the microbial ecology, physical structure and chemical characteristics of biofilms. Consequently, opportunities for the exploitation and control of biofilms were very limited. The past decade has witnessed the development of several new techniques to elucidate the structure and function of biofilms. Examples include: the use of molecular probes that identify different microbes in complex communities as well as their metabolic functions; the use of microsensors that show concentration gradients of key nutrients and chemicals; the use of confocal laser scanning microscopy to describe the physical structure of biofilms and the development of a new generation of mathematical models that allow for the prediction of biofilm structure and function. However, much progress remains to be made in efforts to understand, control and exploit biofilms. This timely book will introduce its readers to the structure and function of biofilms at a fundamental level as determined during the past decade of research, including: Extracellular polymers as the biofilm matrix; Biofilm phenotype (differential gene expression, interspecies signalling); Biofilm ecology; Biofilm monitoring; Resistance of biofilms to antimicrobial agents and Biofilm abatement. Biofilms in Medicine, Industry and Environmental Technology offers a holistic and multi-disciplinary description of the topic, including biofilm formation and composition, but also biofilm monitoring, disinfection and control. All these aspects are presented from three points of views: medical, industrial and environmental biotechnological in a compact, easy to read format.

Biofilms:

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Publisher :
ISBN 13 : 9781685070625
Total Pages : 0 pages
Book Rating : 4.0/5 (76 download)

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Book Synopsis Biofilms: by : Shane Rowland

Download or read book Biofilms: written by Shane Rowland and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Biofilms are naturally occurring clusters of microorganisms that stick to non-biological surfaces, like rocks in a stream. This book contains eight chapters that examine biofilms from a variety of perspectives, including the latest research in this field. Chapter One comprehensively studies the role of endophytic microbes as a potential and alternative source of antimicrobial and antibiofilm bioactive components. Chapter Two discusses how to manage oral microbial biofilm using chemical and herbal medicine. Chapter Three highlights the importance of marine biofouling and the role of coccoid cyanobacteria in this process. Chapter Four focuses on biofilm development, its impact on human health and the problems that are associated with biofilm control. Chapter Five examines microbial biofilms and their role in the environment including agriculture and bioremediation. Chapter Six reviews natural terpenoids and provides descriptions of their structural origin, biological roles and multifunctional properties, such as promoting activity on health-beneficial bacteria. Chapter Seven explains the concepts of biofilm development and the importance of honey and its implications in human health and disease control. Chapter Eight thoroughly studies the potential of honey as antibiofilm, anti-quorum sensing and dispersal agent"--

Biofilms

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Publisher : CRC Press
ISBN 13 : 9781566768696
Total Pages : 270 pages
Book Rating : 4.7/5 (686 download)

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Book Synopsis Biofilms by : Hans-Curt Flemming

Download or read book Biofilms written by Hans-Curt Flemming and published by CRC Press. This book was released on 2000-06-23 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, techniques for studying biofilms- the cellular colonies that live in drinking water systems, wastewater operations, even ground and surface water- have been limited. Yet during the last decade, biofilms have become a critical element in water quality preservation systems, a key component of wastewater treatment biological reactions and the subject of extensive microbiological inquiry. An understanding of biofilm development, structure and dynamics is one condition for improving water supplies and for addressing technical problems such as biofouling, corrosion and bioweathering. Biofilms: Investigative Methods and Applications provides the first in-depth assessment of current and experimental ways to study biofilms, both in sample form and in situ. It shows how sensors, microscopy, lasers, and calorimetry, among other tools, can be used to obtain data on the morphology and metabolism of biofilms. This text is the first to organize and examine the best methods for investigating biofilms. It covers culture-based methods and emerging nondestructive techniques. It also shows how they can be used to characterize biofilms in a variety of manmade settings, such as sewers, wastewater plants, and drinking water distribution systems, as well as in karsts and groundwater sources. In clarifying the way biofilms are studied, the book offers new insights into these living films. It also applies inquiry techniques to the many problems confronting the environmental specialist-corrosion control, biofouling, and the improvement of fixed reactors in wastewater treatment. At the same time it explains technologies for the controlled growth of biofilms and shows how biofilms can be effectively monitored and subjected to quantitative analysis. The technical information in Biofilms: Investigative Methods and Applications is designed to be of use to engineers and researchers and to be helpful in the generation of electronic data.

Biofilms in the Food Environment

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Publisher : John Wiley & Sons
ISBN 13 : 0470276495
Total Pages : 210 pages
Book Rating : 4.4/5 (72 download)

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Book Synopsis Biofilms in the Food Environment by : Hans P. Blaschek

Download or read book Biofilms in the Food Environment written by Hans P. Blaschek and published by John Wiley & Sons. This book was released on 2008-02-28 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofilms in the Food Environment examines biofilms produced by food-borne microorganisms, the risks associated with biofilms in the food chain, the beneficial applications of biofilms in the food environment, and approaches for biofilm removal to improve sanitation and safety in the food environment. Specifically, this book provides: an introduction into the emerging and exciting field of biofilm research in the food environment a summary of advanced knowledge in medical microbiology and engineering and its applicability to food biofilm research, and potential directions for biofilm intervention and industrial beneficial applications that may have direct impact on food safety and public health. Biofilms in the Food Environment is intended to serve as a comprehensive reference source for the food science community, including industry scientists, university researchers, and regulatory agencies. Not only are general concepts regarding biofilms in the food environment covered, but also included are in-depth reviews on biofilm structures, the correlation between strain virulence and biofilm-forming abilities, cutting-edge technologies to investigate microbial compositions in ecosystems and cell-to-cell interactions, and updated findings on molecular attributes and mechanisms involved in biofilm development that might lead to targeted approaches for biofilm prevention and removal. The topics covered and approaches discussed are truly interdisciplinary in nature.