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Application Of Advanced Liquid Chromatography Mass Spectrometry To Cancer And Biopharmaceutical Proteomics Research
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Book Synopsis Application of Advanced Liquid Chromatography Mass Spectrometry to Cancer and Biopharmaceutical Proteomics Research by : Michael Henry
Download or read book Application of Advanced Liquid Chromatography Mass Spectrometry to Cancer and Biopharmaceutical Proteomics Research written by Michael Henry and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advancements of Mass Spectrometry in Biomedical Research by : Alisa G. Woods
Download or read book Advancements of Mass Spectrometry in Biomedical Research written by Alisa G. Woods and published by Springer. This book was released on 2019-07-25 with total page 813 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease, and psychiatry. Additional chapters focus on methodology, technologies and instrumentation, as well as on analysis of protein-protein interactions, protein quantitation, and protein post-translational modifications. Various omics fields such as proteomics, metabolomics, glycomics, lipidomics, and adductomics are also covered. Applications of mass spectrometry in biotechnological and pharmaceutical industry are also discussed. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research, but also to initiate and improve their own work. This book acts as a technical guide as well as a conceptual guide to the newest information in this exciting field.
Book Synopsis Development and Application of Quantitative Liquid Chromatography - Mass Spectrometry Techniques to Facilitate Enhanced Bioprocessing of Therapeutic Proteins by : Amy Farrell
Download or read book Development and Application of Quantitative Liquid Chromatography - Mass Spectrometry Techniques to Facilitate Enhanced Bioprocessing of Therapeutic Proteins written by Amy Farrell and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biopharmaceutical manufacturing is currently the principal growth sector within the pharmaceutical industry. Predominantly formed in mammalian cells, these therapeutic proteins exist as a spectrum of different isoforms and hence pose a unique set of challenges that must be addressed to ensure optimum product quality. To better understand whether the product really is the process, quantitative peptide centred multidimensional liquid chromatography tandem mass spectrometry (LC-MS) based proteomics studies were performed using a Chinese Hamster Ovary (CHO) cell line that expressed an anti-Interleukin-8 IgG1 monoclonal antibody (mAb). Following serum free suspension batch culture under varied bioprocess conditions, quantitative proteomics was completed on the producer CHO cells to elucidate the cellular response to altered culture conditions of pH, temperature and dissolved oxygen. The developed platform was also applied for the analysis of naïve CHO K1 cells following their exposure to spent culture media from the various production runs. Complete characterisation of the expressed mAb was performed, using advanced LC-MS methods including high resolution middle down mass spectrometry; intact protein analysis of critical quality attributes, stable isotope based quantitative glycan analysis and hydrogen deuterium exchange mass spectrometry for structural comparability analysis. In addition data independent LC-MS quantitative proteomics of residual host cell protein impurities was also carried out to evaluate the effect of downstream processing on the quality of the final drug substance. Combined, the findings herein provide a holistic insight into the effect of various upstream and downstream parameters on the quality of therapeutic proteins and facilitate a greater understanding of the molecular mechanisms governing biopharmaceutical production systems, thereby creating a hypothesis for improved future cell line development using various engineering strategies.
Book Synopsis Mass Spectrometry by : Ganesh Shamrao Kamble
Download or read book Mass Spectrometry written by Ganesh Shamrao Kamble and published by BoD – Books on Demand. This book was released on 2019-11-13 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the emerging techniques and applications of mass spectrophotometry in various fields of sustainable chemistry. At the current level of development, the book provides "real-life" examples of analyses and applications. All the chapters are written by internationally recognized authors, and present qualitative and quantitative applications of mass spectrometry. These multidisciplined chapters cover the fundamentals as well as recent advanced analytical techniques, and offer significant information for various researchers studying biomedical, pharmaceutical, and environmental analysis.
Book Synopsis Mass Spectrometry–Based Glycoproteomics and Its Clinic Application by : Haojie Lu
Download or read book Mass Spectrometry–Based Glycoproteomics and Its Clinic Application written by Haojie Lu and published by CRC Press. This book was released on 2021-09-20 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: As one of the most extensive and important protein post-translational modifications, glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently, researchers have focused on the need to characterize protein glycosylation sites, structures, and their degree of modification, to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative, quantitative, and data analysis, together with their clinical applications. Significant examples are discussed to illustrate the principles, laboratory protocols, and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine. Editor: Haojie Lu is a professor at Fudan University, specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.
Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao
Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-09-04 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.
Book Synopsis Protein Analysis using Mass Spectrometry by : Mike S. Lee
Download or read book Protein Analysis using Mass Spectrometry written by Mike S. Lee and published by John Wiley & Sons. This book was released on 2017-05-26 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents Practical Applications of Mass Spectrometry for Protein Analysis and Covers Their Impact on Accelerating Drug Discovery and Development Covers both qualitative and quantitative aspects of Mass Spectrometry protein analysis in drug discovery Principles, Instrumentation, Technologies topics include MS of peptides, proteins, and ADCs , instrumentation in protein analysis, nanospray technology in MS protein analysis, and automation in MS protein analysis Details emerging areas from drug monitoring to patient care such as Identification and validation of biomarkers for cancer, targeted MS approaches for biomarker validation, biomarker discovery, and regulatory perspectives Brings together the most current advances in the mass spectrometry technology and related method in protein analysis
Book Synopsis Applications of Liquid Chromatography-Mass Spectrometry in Bioassays by : Dhananjay D. Shinde
Download or read book Applications of Liquid Chromatography-Mass Spectrometry in Bioassays written by Dhananjay D. Shinde and published by LAP Lambert Academic Publishing. This book was released on 2012-05 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drug discovery is a complex and interdisciplinary research, often with conflicting goals and a complex large data used for the development of a balanced clinical candidate. Written from the perspective of drug discovery, it is a practical guide for the analyst and ADME scientists and advanced students in this field. Separate chapters discuss each important aspect, covering the fundamental concepts of separation science, mass spectrometry and pharmacokinetic (PK) studies discussed with minimum mathematical equations. The present book provides in-hand solutions for offline and online sample extraction methods, column switching techniques, chiral separations and validation of the optimized methods as per the standard protocol and FDA guidelines. With practical details obtained in analytical laboratory...synthesis of drug metabolites and related substances, their characterization, separation, validation and applications in PK studies, book provides a successful approach to design, planning, execution and interpretation of research studies. This book will serve as a practical reference in regular use for researchers and scientist interested in drug discovery.
Book Synopsis Application of Liquid Chromatography-mass Spectrometry and Differential Mobility Spectrometry-mass Spectrometry for Detection and Quantitation of Biomarkers in Complex Biological Matrices by : Zhidan Chen
Download or read book Application of Liquid Chromatography-mass Spectrometry and Differential Mobility Spectrometry-mass Spectrometry for Detection and Quantitation of Biomarkers in Complex Biological Matrices written by Zhidan Chen and published by . This book was released on 2017 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: The projects and discussions in this dissertation are about utilizing modern separation technologies coupled with mass spectrometry for detection and quantitation of various biomarkers in complex biological matrices. Based on the differences of separation phases, the separation technologies in this dissertation will be divided into two directions: Differential Mobility Spectrometry (DMS, one of the ion mobility technologies) in gas phase and Liquid Chromatography (LC) in liquid phase. The projects and applications of each technology will be discussed separately. However, despite the differences of DMS and LC, they can serve as a complementary method for each other. Together, the combination of Ion Mobility technology with Liquid Chromatography technology nowadays have been widely used in both modern life science industry and academia, and will continue being utilized as the workhorse technologies in the near future. This dissertation is mainly divided into three parts: Part I is about the utility of Differential Mobility Spectrometry-Mass Spectrometry (DMS-MS) in radiation biodosimetry. Part II is about the utility of Liquid Chromatography-Mass Spectrometry (LC-MS) in DNA adducts analysis. There are three chapters in each part. Chapter 1-3 are for part I, Chapter 4-6 are for part II. Part III is the conclusions and future directions for this dissertation. Chapter 1 is a relatively short but well-covered introduction about the background of the first project, DMS-MS with its application in biodosimetry. Four major types of ion mobility technologies including drift tube, travelling wave, trapped ion mobility and DMS/FAIMS are summarized and compared in this chapter, with DMS being the focus. Then the history of the development of DMS technology and its development and applications in Vouros lab are introduced, followed with the background of its application in biodosimetry as a rapid and high-throughput screening technology. In chapter 2, a preliminary study of utilizing DMS-MS for rapid and high-throughput detection and quantitation of radiation biomarkers in nonhuman primate urine is shown. In this study, we explored the capability of DMS-MS as an alternative platform for rapid assessment of radiation exposure since the traditional LC-MS platform is too slow due to the nature of chromatography and thus cannot be used for high-throughput analysis purposes. Two radiation biomarkers hypoxanthine and trimethyllysine were chosen for the analysis. The calibration curves and validation tests for each compound were built within 2 hours which is about 10-20 times faster than LC-MS. The level of hypoxanthine in control and 10 Gy radiated group of nonhuman primate urine was determined by this DMS-MS platform and showed a dose response similar to the one obtained by LC-MS but in a much faster manner. This preliminary study has shown that our DMS-MS platform is capable of doing in-field and in-clinic rapid screening of radiation exposure. In chapter 3, as a continuing study of chapter 2, we applied DMS-MS method for simultaneously monitoring multiple radiation biomarkers rather than a single biomarker as shown in the preliminary study in order to achieve better and more confident assessment of radiation exposure. The calibration curves and dose response experiments for all the biomarkers in this study were conducted and showed a much faster throughput than LC-MS as also shown in chapter 2. In chapter 4, the background of DNA adducts and its relationship with cancer, followed by an introduction of using LC-MS as the tool for analysis of DNA adducts, especially the DNA adduct dG-C8-4-ABP in bladder cancer, are introduced. Different mass analyzers and scanning modes are also included in this chapter to give a more detailed information about how to conduct DNA adducts screening. In chapter 5, a relatively detailed review of recent technical and biological development in the analysis of the DNA adduct dG-C8-4-ABP is shown, which allows for a more comprehensive understanding of the whole process of the analysis of DNA adducts, the carcinogenesis of 4-ABP, different methods that can be used for detection and quantitation of dG-C8-4-ABP or other DNA adducts and their comparisons. This chapter is only focused on this single DNA adduct since it is one of the most studied DNA adducts in our laboratory, but the information applies to any other kind of adducts. The different methods of analyzing dG-C8-4-ABP will be covered and discussed thoroughly, from the traditional ℗đ℗øP-postlabelling to the most recent nanoLC-MS and UPLC-MS. In the end, there is a very short introduction about the gender disparity in bladder cancer which is potentially related with hepatic Sulfotransferase (Sult) enzymes. This is a connection linked to chapter 6 which presented a detailed search for the key Sult enzyme/enzymes that are responsible for gender disparity in bladder cancer, and its long-term implications. In chapter 6, as mentioned above, a thorough investigation about the role of hepatic Sult enzymes in the formation of dG-C8-4-ABP in both liver and bladder is conducted. The study is relied on the hypothesis that the androgen can down-regulate some key enzymes which are responsible for activating 4-ABP in the liver, and thus cause less formation of dG-C8-4-ABP in the liver and more in the bladder. This can eventually cause the higher incidence of bladder cancer in men than in women (gender disparity). The purpose of this project is to try to find out which are the key enzymes in the activating process. Previous studies have already excluded most of the candidate enzyme families but left enzyme Sulfotransferase which has several isoforms as our last hope. Only one sult isoform sult1d1 is shown to be positive in in-vitro experiments but unfortunately it was shown to be negative in in-vivo (knock-out) experiments. Other unknown Sult isoforms therefore could be involved with the gender disparity and waiting to be discovered. In Part III, a brief conclusion of the two projects in this dissertation is shown and followed with some expectations and future directions for both the use of DMS-MS in biodosimetry areas and the analysis of DNA adducts.
Book Synopsis Medical Applications of Mass Spectrometry by : Karoly Vekey
Download or read book Medical Applications of Mass Spectrometry written by Karoly Vekey and published by Elsevier Science. This book was released on 2007-11-20 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry is fast becoming an indispensable field for medical professionals. The mass spectrometric analysis of metabolites and proteins promises to revolutionize medical research and clinical diagnostics. As this technology rapidly enters the medical field, practicing professionals and students need to prepare to take full advantage of its capabilities. Medical Applications of Mass Spectrometry addresses the key issues in the medical applications of mass spectrometry at the level appropriate for the intended readership. It will go a long way to help the utilization of mass spectrometry in medicine. The book comprises five parts. A general overview is followed by a description of the basic sampling and separation methods in analytical chemistry. In the second part a solid foundation in mass spectrometry and modern techniques of data analysis is presented. The third part explains how mass spectrometry is used in exploring various classes of biomolecules, including proteins and lipids. In the fourth section mass spectrometry is introduced as a diagnostic tool in clinical treatment, infectious pathogen research, neonatal diagnostics, cancer, brain and allergy research, as well as in various fields of medicine: cardiology, pulmonology, neurology, psychiatric diseases, hemato-oncology, urologic diseases, gastrointestinal diseases, gynecology and pediatrics. The fifth part covers emerging applications in biomarker discovery and in mass spectrometric imaging. * Provides a broad look at how the medical field is benefiting from advances in mass spectrometry. * Guides the reader from basic principles and methods to cutting edge applications. * There is NO comparable book on the market to fill this fast growing field.
Book Synopsis LC-MS/MS in Proteomics by : Pedro R. Cutillas
Download or read book LC-MS/MS in Proteomics written by Pedro R. Cutillas and published by Humana Press. This book was released on 2011-04-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.
Book Synopsis Activity-Based Protein Profiling by : Benjamin F. Cravatt
Download or read book Activity-Based Protein Profiling written by Benjamin F. Cravatt and published by Springer. This book was released on 2019-01-25 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.
Book Synopsis Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics by : Allan Matte
Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Book Synopsis Plant Metabolomics by : Kazuki Saito
Download or read book Plant Metabolomics written by Kazuki Saito and published by Springer Science & Business Media. This book was released on 2006-06-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields.
Download or read book Peptidomics written by Mikhail Soloviev and published by John Wiley & Sons. This book was released on 2007-12-21 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive guide to peptidomics- a hands-on lab reference The first truly comprehensive book about peptidomics for protein and peptide analysis, this reference provides a detailed description of the hows and whys of peptidomics and how the techniques have evolved. With chapters contributed by leading experts, it covers naturally occurring peptides, peptidomics methods and new developments, and the peptidomics approach to biomarker discovery. Explaining both the principles and the applications, Peptidomics: Methods and Applications: * Features examples of applications in diverse fields, including pharmaceutical science, toxicity biomarkers, and neuroscience * Details the successful peptidomic analyses of biological material ranging from plants to mammals * Describes a cross section of analytical techniques, including traditional methodologies, emerging trends, and new techniques for high throughput approaches An enlightening reference for experienced professionals, this book is sufficiently detailed to serve as a step-by-step guide for beginning researchers and an excellent resource for students taking biotechnology and proteomics courses. It is an invaluable reference for protein chemists and biochemists, professionals and researchers in drug and biopharmaceutical development, analytical and bioanalytical chemists, toxicologists, and others.
Book Synopsis Ion Mobility-Mass Spectrometry by : Alison E Ashcroft
Download or read book Ion Mobility-Mass Spectrometry written by Alison E Ashcroft and published by Royal Society of Chemistry. This book was released on 2021-11-11 with total page 621 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, the use of ion mobility separation in combination with mass spectrometry analysis has developed significantly. This technique adds a unique extra dimension enabling the in-depth analysis of a wide range of complex samples in the areas of the chemical and biological sciences. Providing a comprehensive guide to the technique, each chapter is written by an internationally recognised expert and with numerous different commercial platforms to choose from, this book will help the end users understand the practicalities of using different instruments for different ion mobility purposes. The first section provides a detailed account of the fundamentals behind the technique and the current range of available instrumentation. The second section focusses on the wide range of applications that have benefitted from ion mobility – mass spectrometry and includes topics taken from current research in the pharmaceutical, metabolomics, glycomics, and structural molecular biology fields. The book is primarily aimed at researchers, appealing to practising chemists and biochemists, as well as those in the pharmaceutical and medical fields.
Book Synopsis Colorectal Cancer Liver Metastases by : Mauro Monteiro Correia
Download or read book Colorectal Cancer Liver Metastases written by Mauro Monteiro Correia and published by Springer Nature. This book was released on 2019-12-28 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: Colorectal cancer is the third most commonly diagnosed condition in oncology, affecting around 1.23 million individuals per year, according to recent statistics. Of these patients, about 50% will develop liver metastases and approximately 20% will present a stage IV disease at diagnosis. These statistics make colorectal liver metastases (CLM) an issue of major importance in current oncology. The area of CLM is subject to great and continuous advances, as its pathophysiologic mechanisms are better understood and more therapeutic and surgical options are developed. Consequently, all professionals involved with the diagnosis, treatment and follow up of CLM should be kept up to date with the latest advances on the field, to provide high standard medical care to their patients. This book is designed to present the state-of-the-art in CLM management and, in doing so, to review the current evidence on CLM, discussing all important topics in the field. Coverage is broad and comprehensive, encompassing introductory topics (history, definitions, epidemiology, etc.), basic science subjects (molecular biology, genetics, dissemination process, etc.) and practical clinical topics (tumor behavior, diagnosis, drug therapy, radiation therapy, surgery, ablation, multidisciplinary teams, etc.). Although comprehensive on the coverage and selection of topics, each chapter is concise and objective, dissecting topics in a practical and direct format. Evidences and recommendations are included. Chapters display a brief introduction of the common knowledge, go straight to the detailed revision of the most recent years of the literature, and end with practical closing observations. This book is a tool for professionals (general and cancer surgeons, HPB surgeons, clinical oncologists, gastroenterologists and medical residents) and interns who search for a qualified and reader friendly revision on topics concerning Colorectal Cancer Liver Metastases.
