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Author : Tianlei Ying
Publisher : Frontiers Media SA
ISBN 13 : 2889456781
Total Pages : 118 pages
Book Rating : 4.8/5 (894 download)
Download or read book Antibody Fc Engineering: Towards Better Therapeutics written by Tianlei Ying and published by Frontiers Media SA. This book was released on 2018-12-21 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (136 download)
Download or read book Antibody Fc Engineering: Towards Better Therapeutics written by and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal antibodies and Fc-fusion proteins used clinically are Fc-based therapeutics that grow fastest in the pharmaceutical industry. Since they both contain an Fc fragment, engineering of Fc fragments could be a platform for improving Fc-based drug efficacy. Fc engineering includes various aspects: stabilization of Fc; regulation of effector functions including antibody-dependent cell-mediated cytotoxicity, complement-dependent cytotoxicity; extension of serum half-life by modification of neonatal Fc receptor (FcRn) binding; monomerization or heterodimerization of Fc for design of new Fc formats. Currently, many new methods are being used in Fc engineering. Compared to traditional methods such as site mutagenesis on certain positions by amino acid replacement, new methods such as display-based technology can confer high throughput screening and obtain optimized variants relatively quickly, accelerating the drug development process. With the new methods, many new Fc variants were identified. On this Research Topic we are going to review the progress in current Fc engineering including the new engineering methods and the Fc variants or constructs they have produced, and the potential of these new Fcs in clinical use.
Author : William R Strohl
Publisher : Elsevier
ISBN 13 : 1908818093
Total Pages : 697 pages
Book Rating : 4.9/5 (88 download)
Download or read book Therapeutic Antibody Engineering written by William R Strohl and published by Elsevier. This book was released on 2012-10-16 with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering. - Goes beyond the standard engineering issues covered by most books and delves into structure-function relationships - Integration of knowledge across all areas of antibody engineering, development, and marketing - Discusses how current and future genetic engineering of cell lines will pave the way for much higher productivity
Author : Margaret Ackerman
Publisher : Academic Press
ISBN 13 : 0123948185
Total Pages : 376 pages
Book Rating : 4.1/5 (239 download)
Download or read book Antibody Fc written by Margaret Ackerman and published by Academic Press. This book was released on 2013-08-06 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibody Fc is the first single text to synthesize the literature on the mechanisms underlying the dramatic variability of antibodies to influence the immune response. The book demonstrates the importance of the Fc domain, including protective mechanisms, effector cell types, genetic data, and variability in Fc domain function. This volume is a critical single-source reference for researchers in vaccine discovery, immunologists, microbiologists, oncologists and protein engineers as well as graduate students in immunology and vaccinology. Antibodies represent the correlate of protection for numerous vaccines and are the most rapidly growing class of drugs, with applications ranging from cancer and infectious disease to autoimmunity. Researchers have long understood the variable domain of antibodies, which are responsible for antigen recognition, and can provide protection by blocking the function of their target antigen. However, recent developments in our understanding of the protection mediated by antibodies have highlighted the critical nature of the antibody constant, or Fc domain, in the biological activity of antibodies. The Fc domain allows antibodies to link the adaptive and innate immune systems, providing specificity to a wide range of innate effector cells. In addition, they provide a feedback loop to regulate the character of the immune response via interactions with B cells and antigen-presenting cells. - Clarifies the different mechanisms of IgG activity at the level of the different model systems used, including human genetic, mouse, and in vitro - Covers the role of antibodies in cancer, infectious disease, and autoimmunity and in the setting of monoclonal antibody therapy as well as naturally raised antibodies - Color illustrations enhance explanations of the immune system
Author : Tae Hyun Kang
Publisher :
ISBN 13 :
Total Pages : 244 pages
Book Rating : 4.:/5 (12 download)
Download or read book Fc Engineering for the Reprogramming the Effector Functions of Antibodies for Improved Therapeutic Potency written by Tae Hyun Kang and published by . This book was released on 2013 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal antibodies (mAb) are very important for cancer therapy, as they target cancerous cells without side effects. mAbsrecognize cell-surface proteins on cancerous cells and mediate the killing of these targeted cells by multiple mechanisms, including antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cell-mediated phagocytosis (ADCP), both of which are dependent upon a fragment crystallizable (Fc) domain interacting with effector Fc gamma receptors (Fc[gamma]Rs). mAb can induce productive synapse formation between tumor and immune effector cells by recruiting Fc[gamma]Rs on the effector cells. N-linked carbohydrate chains on the Fc domain of an immunoglobulin (Ig)G1 antibodies are so critical for binding to Fc[gamma]Rs that the antibodies cannot measurably bind to the receptors without the glycans. However, we have recently isolated an engineered aglycosylated Fc variant with selectivity towards Fc[gamma]RI with nanomolar K[subscript D] but no detectable binding to other Fc[gamma]Rs. This Fc-engineered aglycosylated antibody shows novel effector functions which do not show in glycosylated counterparts. The novel property of the engineered antibody is derived from selective binding to one or more of the activating Fc[gamma]Rs but not to the inhibitory Fc[gamma]RIIb, which mediates potent inhibition of cellular immune activity. This unique effector function indicates that aglycosylated IgGs can be reprogrammed to display unique Fc[gamma]R selectivity profiles. These Fc-engineered aglycosylated antibodies have advantages over glycosylated antibodies because they can be produced in bacteria at a low cost through rapid bioprocessing while circumventing the problem of glycan heterogeneity. This dissertationdescribes engineering of aglycosylated antibodies that selectively binds to i) Fc[gamma]RIIIa and also Fc[gamma]RI (Chapter 2) and ii) bind to Fc[gamma]RIIIa but show decreased or no detectable binding to the inhibitory receptor Fc[gamma]RIIb (Chapter 3). BIACore analysis of the binding of the engineered Fc domains to recombinant Fc[gamma]Rs is described in detail. This dissertation also describes a screening strategy for pH-dependent FcRn binding (Chapter 4). In separate studies, binding properties of Fc5 to Fc[gamma]RI using Fc-III peptideand its implication on structure as well as affinities of Fc1000 serieswere evaluated (Appendices A and B, respectively).
Author : Allan Matte
Publisher : Elsevier
ISBN 13 : 0081030193
Total Pages : 222 pages
Book Rating : 4.0/5 (81 download)
Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Author : William James Kelton
Publisher :
ISBN 13 :
Total Pages : 326 pages
Book Rating : 4.:/5 (93 download)
Download or read book Engineering Antibody Fc Domains for Improved Therapeutic Function written by William James Kelton and published by . This book was released on 2013 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Therapeutic antibodies have achieved exceptional clinical success in the treatment of cancer and other human diseases. Now, new approaches are required to enhance the potency of antibodies to further increase the number of patients responding to therapy. By engineering the antibody Fc domain through mutation of the amino acid sequence, binding affinity to activating or inhibitory Fc receptors on effector cells can be increased to modulate the cellular immune response. However, attaining selectivity for closely related Fc receptors has proved challenging and the technique has not been applied to access the function of antibody isotypes other than IgG. Here we present new methods for enhancing antibody potency using both hybrid IgA/G and aglycosylated Fc domains. In the first instance, a chimeric antibody Fc domain has been created by combining residues from IgA with those from IgG. The new variant, MutD, introduces binding to Fc [alpha] RI while retaining affinity for certain members of the Fc [gamma] R family. ADCC assays show MutD, when part of a full length trastuzumab antibody against Her2 antigen, can kill Her2-overexpressing tumor cell lines as effectively as IgA antibodies. Moreover, MutD shows improved assembly compared to IgA and thus provides access to potent Fc [ alpha] RI function while overcoming the expression and purification barriers that have limited the use of IgA as a therapeutic. Alternatively, aglycosylated antibodies may be engineered for exceptional effector function. Glycans anchored to residue N297 of the antibody IgG Fc domain are typically critical in mediating binding toward the Fc [gamma]Rs. Yet, using a full length bacterial IgG display system, we have isolated aglycosylated Fc1004 with mutations that confer a 160-fold increase in the affinity toward the low affinity Fc [gamma] RIIa-R131 allele as well as high selectivity against binding to the remarkably homologous inhibitory receptor, Fc [gamma] RIIb. Incorporation of this engineered Fc into trastuzumab resulted in a 75% increase in tumor cell phagocytosis by macrophages compared to that of the parental glycosylated trastuzumab with medium Her2-expressing cancer cells. In vivo testing of Fc1004 using NOD/SCID mouse model, reconstituted by adoptive transfer of leukocytes from Fc [gamma] RIIa-R131 homozygous donors, showed a promising reduction in tumor burden in SkBr-3 Her2+ xenografts.
Author : Zhiqiang An
Publisher : John Wiley & Sons
ISBN 13 : 1118210263
Total Pages : 932 pages
Book Rating : 4.1/5 (182 download)
Download or read book Therapeutic Monoclonal Antibodies written by Zhiqiang An and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 932 pages. Available in PDF, EPUB and Kindle. Book excerpt: 70-chapter authoritative reference that covers therapeutic monoclonal antibody discovery, development, and clinical applications while incorporating principles, experimental data, and methodologies. First book to address the discovery and development of antibody therapeutics in their entirety. Most chapters contain experimental data to illustrate the principles described in them. Authors provide detailed methodologies that readers can take away with them and use in their own laboratories.
Author : Margaret Ackerman
Publisher :
ISBN 13 : 9780123948021
Total Pages : 363 pages
Book Rating : 4.9/5 (48 download)
Download or read book Antibody Fc written by Margaret Ackerman and published by . This book was released on 2013 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book synthesizes the literature on the mechanisms underlying the dramatic variability of antibodies to influence the immune response. The book demonstrates the importance of the Fc domain, including protective mechanisms, effector cell types, genetic data, and variability in Fc domain function.
Author : Stefan Dübel
Publisher : John Wiley & Sons
ISBN 13 : 3527329374
Total Pages : 2538 pages
Book Rating : 4.5/5 (273 download)
Download or read book Handbook of Therapeutic Antibodies written by Stefan Dübel and published by John Wiley & Sons. This book was released on 2014-12-03 with total page 2538 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dieses Nachschlagewerk zu therapeutischen Antikörpern sucht auch in der komplett überarbeiteten 2. Auflage seinesgleichen und bietet 30 % neue Inhalte zu Entwicklung, Herstellung und therapeutischen Anwendungen dieser Biomoleküle.
Author : Huimin Zhao
Publisher : John Wiley & Sons
ISBN 13 : 3527344705
Total Pages : 41 pages
Book Rating : 4.5/5 (273 download)
Download or read book Protein Engineering written by Huimin Zhao and published by John Wiley & Sons. This book was released on 2021-08-23 with total page 41 pages. Available in PDF, EPUB and Kindle. Book excerpt: A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.
Author : Damien Nevoltris
Publisher : Humana
ISBN 13 : 9781493993536
Total Pages : 543 pages
Book Rating : 4.9/5 (935 download)
Download or read book Antibody Engineering written by Damien Nevoltris and published by Humana. This book was released on 2019-12-10 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.
Author : Steven M. Chamow
Publisher : John Wiley & Sons
ISBN 13 : 3527675280
Total Pages : 345 pages
Book Rating : 4.5/5 (276 download)
Download or read book Therapeutic Fc-Fusion Proteins written by Steven M. Chamow and published by John Wiley & Sons. This book was released on 2013-12-18 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited by three pioneers in the field, each with longstanding experience in the biotech industry, and a skilled scientific writer, this is the first book to cover every step in the development and production of immunoglobulin Fc-fusion proteins as therapeutics for human disease: from choosing the right molecular design, to pre-clinical characterization of the purified product, through to batch optimization and quality control for large-scale cGMP production. The whole of the second part is devoted to case studies of Fc-fusion proteins that are now commercially successful products. In this section, the authors, several of whom were personally involved in clinical development of the products themselves, detail the product?s background and give insight into issues that were faced and how these issues were overcome during clinical development. This section also includes a chapter on promising new developments for the future. An invaluable resource for professionals already working on Fc-fusion proteins and an excellent and thorough introduction for physicians, researchers, and students entering the field.
Author : Roohi Bansal
Publisher : Roohi Bansal
ISBN 13 : 9789355781604
Total Pages : 390 pages
Book Rating : 4.7/5 (816 download)
Download or read book Antibodies and Their Role in Therapeutics written by Roohi Bansal and published by Roohi Bansal. This book was released on 2021-12-04 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the structure, types, functions, and gene organization of antibodies, the book aims to shine a detailed light on the monoclonal antibodies (often referred to as mAbs) that have revolutionized the fields of therapeutics and diagnostics. The book describes the different ways of generating chimeric, humanized, and fully human monoclonal antibodies, emphasizing phage display, hybridoma, and rDNA technology. In addition, the book focuses on the various recombinant antibody formats in detail: Drug conjugates: Antibody-drug conjugates (ADCs), Immunotoxins (Recombinant, Humanized and Fully Human) and Antibody-antibiotic conjugate (AAC) Bispecific antibodies: scFv based (BiTE, DARTs and TandAbs) and Full-length IgG based Abzymes and Antibody-directed enzyme prodrug therapy (ADEPT) Fc-fusion proteins Single-domain antibodies (VHH and IgNAR sdAb) The book discusses the various therapeutic applications of monoclonal antibodies, along with the immunogenicity issues. The book also covers the modes of administration and side effects of monoclonal antibodies, along with the challenges and issues faced while developing a monoclonal antibody into a therapeutic agent. Modifications introduced by the researchers to decrease the immunogenicity issues and increase the efficacy of therapeutic mAbs are also described. The book is an invaluable resource for researchers and students in biology and medicine, biotechnology, immunology, genetics, molecular biology, and anyone interested in antibody engineering.
Author : Clive R. Wood
Publisher : World Scientific
ISBN 13 : 1848166281
Total Pages : 490 pages
Book Rating : 4.8/5 (481 download)
Download or read book Antibody Drug Discovery written by Clive R. Wood and published by World Scientific. This book was released on 2012 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibody-based therapeutics are a central driver of the success of biopharmaceuticals. The discovery technology of this field is isolated to a limited number of centers of excellence in industry and academia. The objective of this volume is to provide a series of guides to those evaluating and preparing to enter particular areas within the field. Each chapter is written with a historical perspective that sets into context the significance of the key developments, and with the provision of “points to consider” for the reader as a value-added feature of the volume. All contributors are experts in their fields and have played pivotal roles in the creation of the technology.
Author : Rozen Le Panse
Publisher : Frontiers Media SA
ISBN 13 : 2889661075
Total Pages : 205 pages
Book Rating : 4.8/5 (896 download)
Download or read book Advances in Autoimmune Myasthenia Gravis written by Rozen Le Panse and published by Frontiers Media SA. This book was released on 2020-10-27 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Author : Cheng Liu
Publisher : John Wiley & Sons
ISBN 13 : 1118940628
Total Pages : 723 pages
Book Rating : 4.1/5 (189 download)
Download or read book Biosimilars of Monoclonal Antibodies written by Cheng Liu and published by John Wiley & Sons. This book was released on 2016-12-09 with total page 723 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addressing a significant need by describing the science and process involved to develop biosimilars of monoclonal antibody (mAb) drugs, this book covers all aspects of biosimilar development: preclinical, clinical, regulatory, manufacturing. • Guides readers through the complex landscape involved with developing biosimilar versions of monoclonal antibody (mAb) drugs • Features flow charts, tables, and figures that clearly illustrate processes and makes the book comprehensible and accessible • Includes a review of FDA-approved mAb drugs as a quick reference to facts and useful information • Examines new technologies and strategies for improving biosimilar mAbs
